Substituted 4,4-difluoro-4-bora-3A,4A-diaza-s-indacene compounds for homogenous amplification/detection assays

摘要

The use of BODIPY TM fluorophores for detecting a target nucleic acid is described. The parent heterocyclic molecule of the BODIPY TM fluorophores is a dipyrrometheneboron difluoride compound which is modified to create a broad class of spectrally-discriminating fluorophores. The present invention provides oligonucleotides labelled with substituted 4,4-difluoro-4-bora-3A,4A-diaza-s-indacene (BODIPY TM fluorophore) compounds for performing the Taqman assay.