发明名称 |
Anti-IgE antibodies and methods of improving polypeptides |
摘要 |
The present invention relates to a method for adjusting the affinity of a polypeptide to a target molecule by a combination of steps, including: (1) the identification of aspartyl residues which are prone to isomerization; (2) the substitution of alternative residues and screening the resulting mutants for affinity against the target molecule. In a preferred embodiment, the method of subtituting residues is affinity maturation with phage display (AMPD). In a further preferred embodiment the polypeptide is an antibody and the target molecule is an antigen. In a further preferred embodiment, the antibody is anti-IgE and the target molecule is IgE. In another embodiment, the invention relates to an anti-IgE antibody having improved affinity to IgE.
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申请公布号 |
US5994511(A) |
申请公布日期 |
1999.11.30 |
申请号 |
US19970887352 |
申请日期 |
1997.07.02 |
申请人 |
GENENTECH, INC. |
发明人 |
LOWMAN, HENRY B.;PRESTA, LEONARD G.;JARDIEU, PAULA M.;LOWE, JOHN |
分类号 |
C12N15/02;A61K38/00;A61K39/395;A61P37/00;A61P37/06;A61P37/08;A61P43/00;C07K16/00;C07K16/42;C12N15/13;C12P21/08;(IPC1-7):A61K39/395 |
主分类号 |
C12N15/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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