OLIGONUCLEOTIDES FOR DETECTING ENTERIC HEMORRHAGIC E. COLI AND DETECTION METHOD USING THE SAME

摘要

The object of the present invention is to provide a simple, rapid and highly sensitive method of examining EHEC or VTEC in the examination of food poisoning and diarrhea. In the present invention, the oligonucleotides of SEQ ID NOS: 1-9 hybridizing selectively with a Vero toxin gene from EHEC (or VTEC) or with an 0 antigen-synthesizing region gene from pathogenic E. coli 0157 are prepared and used as primers for gene amplification. By this method, bacteria producing 0157 as one of the pathogenic factors in these bacteria and E. coli capable of producing Vero toxin can be selectively detected.