| 摘要 |
A nucleic acid hybridization process is provided which employs a modified oligonucleotide and improves the ability to discriminate a control nucleic acid target from a variant nucleic acid target containing a sequence variation. The modified probe contains at least one artificial mismatch relative to the control nucleic acid target in addition to any mismatch(es) arising from the sequence variation. This has direct and advantageous application to numerous existing hybridization methods including applications that employ, for example, the polymerase chain reaction, allele-specific nucleic acid sequencing methods, and diagnostic hybridization methods
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