| 摘要 |
The present invention describes introducing metal ion affinity sequences into recombinant proteins to allow for purification and/or immobilization of these proteins. The present invention provides a fusion protein, comprising: a protein of interest fused at its amino-terminus or carboxy-terminus to at least one affinity peptide, said fusion protein having a formula R1-(HXn)m-R2, wherein R1 or R2 is said protein of interest, n = 1-8, m = 2-30, and wherein if n = 1 for more than two adjacent units of HX, at least one X must be asparagine, phenylalanine, tryptophan, tyrosine, lysine, methionine, arginine, glutamine, or cysteine. |
