Transcriptional control sequences which regulate gene expression in plant tissue in response to invading microbial pathogens and elicitor inducing chemical signals

摘要

Using a DNA molecule including a nucleotide sequence encoding an elicitin protein operably linked to and expressed under the regulatory control of an inducible transcription regulatory element to produce a transgenic plant with improved disease resistance includes providing a nucleotide sequence encoding a heterologous coding sequence of an elicitin protein operably linked to an inducible transcription regulatory element and a promoter functional in a plant cell to produce an inducible expression construct. The construct is cloned into a plasmid vector adapted for use in a plant cell to produce an inducible expression vector which is introduced into plant tissue to produce transgenic plant tissue. The transgenic plant tissue is regenerated to produce a transgenic plant in which the elicitin is expressed in response to potential plant pathogen invasion or elicitor treatment of the transgenic plant to induce the hypersensitive response increasing the resistance of the transgenic plant to the plant pathogen.