摘要 |
PROBLEM TO BE SOLVED: To obtain a member which enhances the separating capability of the electrophoresis of a microchip. SOLUTION: In order to guide a sample to a crossing part 12 on the surface of a baseplate 4 at a microchip 2, a voltage of 1.2 kV is applied to reservoirs 18a to 18c, 18f, a voltage of 1.0 kV is applied to a reservoir 18e, and a reservoir 18d is grounded. At this time, the potential of the crossing part 12 becomes about 0.87 V. A sheath flow which is shown in (C), (D) is formed in a sample flow passage 10 by an electroosmotic flow. The sample is guided to the crossing part 12 along a slanted line part (the central part of the sheath flow). A voltage of 1.0 kV is applied to the reservoir 18d so that the sample which is guided to the crossing part 12 is guided to an analytical flow passage 8, and the reservoir 18f is grounded. In order to prevent the sample in an excessive amount from flowing into from the sample flow passage 10, a voltage of 0.5 kV is applied to the reservoirs 18a to 18c, 18d. In this manner, a thin sample plug can be introduced into the analytical flow passage 8 as shown in (E). |