发明名称 |
METHODS FOR PRODUCING A POLYPEPTIDE IN A BACILLUS CELL |
摘要 |
The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and alternatively also (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a "consensus" promoter having the sequence TTGACA for the "-35" region and TATAAT for the "-10" region operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and (ii) an mRNA processing/stabilizing sequence located downstream of the "consensus" promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium. |
申请公布号 |
WO9943835(A3) |
申请公布日期 |
1999.11.25 |
申请号 |
WO1999US04360 |
申请日期 |
1999.02.26 |
申请人 |
NOVO NORDISK BIOTECH, INC. |
发明人 |
WIDNER, WILLIAM;SLOMA, ALAN;THOMAS, MICHAEL, D. |
分类号 |
C12N15/09;C07K14/325;C12N1/21;C12N9/54;C12N15/32;C12N15/75;C12R1/07 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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