发明名称 |
GENERATION OF MULTIPLE GENE PRODUCTS FROM CHIMERIC FUSION PROTEINS BY CLEAVAGE WITH UBIQUITOUS ENDOPROTEASES |
摘要 |
The present invention concerns materials and methods relating to the expression of genes. In particular, it relates to the expression of multiple gene products as a single cistron. The invention is achieved by constructing a nucleic acid construct comprising a first sequence encoding a first polypeptide (such as a protein subunit) and a second sequence encoding a second polypeptide (a further protein subunit), said first and second sequences being separated by a linker sequence encoding an endoprotease cleavage site. Once expressed as a single fusion protein it can be cleaved a nd processed into its constituent, biologically active, first and second polypeptides. The invention has particular implications with regard to expression of proteins that have multiple subunits (e.g. IL-12) that require expression of the independent subunits at equimolar amounts.
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申请公布号 |
CA2333144(A1) |
申请公布日期 |
1999.11.25 |
申请号 |
CA19992333144 |
申请日期 |
1999.05.21 |
申请人 |
CANCER RESEARCH VENTURES LIMITED |
发明人 |
GAKEN, JOHANNES ADRIANUS;RUSSELL, STEPHEN JAMES;FARZANEH, FARZIN |
分类号 |
C12N15/09;A61K39/00;A61K48/00;A61P37/04;C07K14/47;C07K14/54;C07K14/55;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12P21/02;(IPC1-7):C12N15/62;C12N15/63 |
主分类号 |
C12N15/09 |
代理机构 |
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主权项 |
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地址 |
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