A PURIFIED POLYNUCLEOTIDE SEQUENCE CONTAINING A REGION ENCODING A MUTATED ZAP-70 PROTEIN OR A FRAGMENT THEREOF

摘要

The present invention relates to a purified polynucleotide containing a sequence encoding the linker region of the ZAP-70 protein, or a fragment thereof, wherein said sequence is mutated at least at one nucleotide located between nucleotide 1047 and nucleotide 1199 (corresponding respectively to amino acid residue 280 and amino acid residue 315). More particularly the mutation is between nucleotide 1155 and nucleotide 1184 (corresponding respectively to amino acid residue 316 and amino acid residue 325) and preferably at position 1064 (corresponding to amino acid residue 319). The present invention also provides a polypeptide corresponding to the polynucleotide according to the invention, and includes a method of screening for molecules capable of modifying the activation of T-cells wherein said molecules can interact positively or negatively on the binding of a ZAP-70 protein and a Src-Homology (SH) 2 domain-containing protein such as the p56 signaling protein. The present invention also relates to active molecules as obtained according to the method of the invention and uses for the nucleic acids, polypeptides, and active molecules of the invention.