发明名称 |
INFECTION AND PRLIFERATION OF VIRUS IN INSECT SUCH AS SILKWORM |
摘要 |
<p>PROBLEM TO BE SOLVED: To provide a method by which a useful substance can efficiently be produced according to safe and simple operations without any fear of environmental pollution with a virus when the virus, etc., containing a gene encoding a useful protein integrated therein are inoculated into an insect such as silkworm to infect the insect therewith and the proliferation thereof is then carried out to mass-produce the useful substance. SOLUTION: A virus inclusion body is purified by a sedimentation velocity centrifugation method and then dissolved in a dilute aqueous solution of sodium carbonate to carry out an enzymic treatment of a liberated virus and sodium iodide is added before precipitation and recovery of a viral DNA with an alcohol when separating and purifying the viral DNA from the virus in a method for promoting the infection and transfer of the viral DNA into a host and proliferating the virus comprising mixing the DNA separated and purified from the virus with an aqueous solution containing a cationic lipid and percutaneously inoculating the DNA into an insect such as silkworm.</p> |
申请公布号 |
JPH11318451(A) |
申请公布日期 |
1999.11.24 |
申请号 |
JP19980153608 |
申请日期 |
1998.05.18 |
申请人 |
NATL INST OF SERICULTURAL & ENTOMOLOGICAL SCIENCE;YONEMURA MASAYUKI |
发明人 |
YONEMURA MASAYUKI |
分类号 |
C12N15/09;C12N7/00;(IPC1-7):C12N15/09 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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