发明名称 |
METHODS FOR ENHANCING NUCLEIC ACID AMPLIFICATION |
摘要 |
A method for amplification of a nucleic acid strand in a test sample. The method includes contacting the nucleic acid strand from the test sample simultaneously with at least three oligonucleotide primers. At least one primer is a promoter-primer, and at least one other primer is complementary to the nucleic acid strand, and one other primer is complementary to a strand complementary to the nucleic acid strand. The method further includes contacting the nucleic acid strand and primers with one or more proteins having RNA-directed and/or DNA-directed DNA polymerase activities, an RNA polymerase activity, and an RNAse H activity under primer-extension conditions to allow amplification of a target region in the nucleic acid strand at essentially constant temperature.
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申请公布号 |
CA2167838(C) |
申请公布日期 |
1999.11.23 |
申请号 |
CA19942167838 |
申请日期 |
1994.07.20 |
申请人 |
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发明人 |
RYDER, THOMAS B.;BILLYARD, ELIZABETH R.;DATTAGUPTA, NANIBHUSHAN |
分类号 |
C12N15/09;C12Q1/68;(IPC1-7):C12Q1/68;C12N15/10;C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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