发明名称 |
Stable transformation of plant cells |
摘要 |
The invention provides methods for producing a fertile, stably transformed, Zea mays plant. The methods comprise the steps of (a) providing a foreign DNA comprising an expression vector carrying a gene encoding an agronomic trait; (b) providing a maize embryogenic callus, suspension culture, or immature embryo isolated from a plant; (c) introducing the foreign DNA into the embryogenic callus, suspension culture or immature embryo isolated from a plant by one or more microparticle bombardments; and (d) regenerating fertile transgenic Zea mays plant.
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申请公布号 |
US5990387(A) |
申请公布日期 |
1999.11.23 |
申请号 |
US19940319352 |
申请日期 |
1994.10.06 |
申请人 |
PIONEER HI-BRED INTERNATIONAL, INC. |
发明人 |
TOMES, DWIGHT T.;WEISSINGER, ARTHUR;SANFORD, JOHN C.;KLEIN, THEODORE M. |
分类号 |
C12N15/29;C12N15/31;C12N15/82;(IPC1-7):C12N15/00;A01H1/06;A01H4/00 |
主分类号 |
C12N15/29 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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