FUSED PROTEIN

摘要

A fused protein containing a target protein which consists of, in the direction from the N-end toward the C-end, a) a signal sequence; b) an immunoglobulin Fc region with the deletion of at least CH1 domain; c) a peptide linker containing at the C-end an enzyme cleavage site allowing cleavage with enterokinase, etc.; and d) the amino acid sequence of the target protein such as erythropoietin, characterized in that, after the completion of the enzymatic cleavage, the target protein contains at the N-end no amino acid residue originating in the peptide linker. Thus, a target protein free from any amino acid-modification at the N-end can be efficiently produced by an enzymatic treatment.