| 摘要 |
This invention is directed to an optically-based method and system for analy te detection using solid phase immobilization, specific analyte labels adapted for signal generation and corresponding processes for the utilization thereo f. The enumeration detection method disclosed herein narrows the area for signa l observation, thus, improving detectable signal to background ratio. The syst em is comprised of a platform/support for immobilizing a sample stage having a labeled sample (analyte complex) bound thereto, a radiation source, an optic al apparatus for generating and directing radiation at said sample and a means for data collection and analysis. Upon engagement of the system, the sample generates a signal capable of differentiation from background signal, both o f which are collected and imaged with a signal detector that generated a sampl e image to a data processing apparatus. Said apparatus receives signal measurements and, in turn, enumerates individual binding events. Generated signal may be increased via selected mass enhancement. The invention, enumeration assay methodology detecting individual binding events, may be used, for example, in analyses to detect analyte or confirm results in both research, commercial and point of care applications.
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