发明名称 |
CONTINUOUS IN VITRO EVOLUTION |
摘要 |
The present invention provides a method for the mutation, synthesis and selection of a protein which binds to a target molecule, the method comprising: (a) incubating a replicable mRNA molecule encoding the protein with ribonucleoside triphosphate precursors of RNA and an RNA-directed RNA polymerase, wherein the RNA-directed RNA polymerase replicates the mRNA molecule but introduces mutations thereby generating a population of mutant mRNA molecules; (b) incubating the mutant mRNA molecules from step (a) with a translation system under conditions which result in the synthesis of a population of mutant proteins such that after translation, mutant proteins are linked to their encoding mRNA molecules thereby forming a population of mutant protein/mRNA complexes; (c) selecting one or more mutant protein/mRNA complex(es) by exposing the population of mutant protein/mRNA complexes from step (b) to the target molecule and recovering the mutant protein/mRNA complex(es) bound thereto.
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申请公布号 |
CA2328515(A1) |
申请公布日期 |
1999.11.18 |
申请号 |
CA19992328515 |
申请日期 |
1999.05.07 |
申请人 |
DIATECH PTY. LTD. |
发明人 |
IRVING, ROBERT ALEXANDER;HUDSON, PETER JOHN;COIA, GREGORY;ILIADES, PETER |
分类号 |
C12N15/09;C07K16/08;C07K16/18;C12N9/12;C12N15/01;C12N15/10;C12P21/02;(IPC1-7):C12N15/01;C12P21/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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