Highly active alkaline phosphatase

摘要

DNA encoding a eukaryotic high active alkali phosphatase with a specific activity over 3000 U/mg where the amino acid at position 332 is smaller than aspartate is new. Independent claims are also included for: (1) a method to produce a DNA as above, characterized in that the mutated and wild type fragments of the cDNA of one or more alkali phosphatases are ligated into a single gene that encodes for an active alkali phosphatase; (2) a eukaryotic cDNA, that encodes a functional isoenzyme with alkali phosphatase activity and which is an intermediate product during the method of (1); (3) a vector containing a cDNA as above; (4) a eukaryotic or prokaryotic cell containing a vector as in (3); (5) a high active recombinant alkali phosphatase as above; (6) a method to produce a high active alkali phosphatase as above; and (7) a native high active alkali phosphatase having one of two 511 amino acid sequences (bIAP II or bIAP IV).