| 摘要 |
PCT No. PCT/EP95/04164 Sec. 371 Date Apr. 23, 1997 Sec. 102(e) Date Apr. 23, 1997 PCT Filed Oct. 24, 1995 PCT Pub. No. WO96/12510 PCT Pub. Date May 2, 1996Epithelial cells are attached to microcarriers to form a transplantation material for treatment of skin wounds. Preferably, keratinocyte-covered microcarriers are prepared using microcarriers having a diameter of 50 to 500 mu m, and obtaining a coverage of keratinocytes of 30 to 100% of the maximum coverage. An optimum coverage is between 50 and 80% and preferably between 60 and 70%. Keratinocytes are selected from autologous cells, allogenic cells and a combination of these cells. The microcarriers may be pre-coated with eukaryotic cells or an extracellular matrix protein such as collagen before keratinocytes are attached. The keratinocyte-covered microcarriers may be combined with a cryoprotective substance for storage at +3 DEG C. to -196 DEG C. prior to use. Keratinocytes are attached to the microcarriers by pre-culturing the keratinocytes, and then culturing the keratinocytes in the presence of the microcarriers while intermittently stirring. A serum free culture medium may be used. Initial culturing in the presence of the microcarriers may be in the smallest amount of medium required to prevent the keratinocytes from dying, and after keratinocyte attachment, adding medium to provide a volume for optimum growth. The keratinocyte-covered microcarriers are applied to a skin wound and the wound is covered with a protective dressing. A pharmaceutical formulation and kit are prepared containing the keratinocyte-covered microcarriers may.
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