发明名称 |
Production of proteins using homologous recombination |
摘要 |
Methods and compositions are provided for expression of mammalian genes in culture. An amplifiable gene is introduced by homologous recombination in juxtaposition to a target gene, the resulting combination of amplifiable gene and target gene transferred to a convenient host and the target gene amplified by means of the amplifiable gene. The resulting expression host may then be grown in culture with enhanced expression of the target gene.
|
申请公布号 |
US5981214(A) |
申请公布日期 |
1999.11.09 |
申请号 |
US19950467244 |
申请日期 |
1995.06.06 |
申请人 |
CELL GENESYS, INC. |
发明人 |
SKOULTCHI, ARTHUR I. |
分类号 |
C12N15/09;C07K14/505;C07K14/535;C07K14/59;C12N1/19;C12N5/10;C12N15/12;C12N15/81;C12N15/85;C12N15/86;C12N15/90;C12P21/00;C12Q1/68;C12R1/91;(IPC1-7):C12N15/00 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|