| 摘要 |
<p>An isolated cephalosporin deacetylase derived from a strain of Bacillu sp. which (i) has an optimum pH between 8.0 and 9.0; (ii) has an optimum temperature of about 40 °C; and (iii) has an isoelectric point of about 4.3, and a DNA sequence which codes for the cephalosporin deacetylase are disclosed. An expression vector comprising the DNA sequence and transformed host strain carrying the vector are provided and used to deacetylate cephalosporin compounds to give useful intermediates for cephalosporin antibiotics synthesis.</p> |
