METHODS OF SELECTING INTERNALIZING ANTIBODIES

摘要

This invention provides methods of selecting antibodies that are internalize d into target cells. The methods generally involve contacting target cells wit h one or more members of an antibody phage display library, shown in the figur e. The members of the phage display library are also contacted with cells of subtractive cell line. The target cells are then washed to remove the subtractive cell line cells and members of phage display library that are no n- specifically bound or weakly bound to the target cells. The target cells are cultured under conditions where members of the phage display library can be internalized if bound to an internalizing marker and internalized members of the phage display library are then identified.