发明名称 |
Obtaining human dendritic cells from monocytes, useful for modifying immune response and for treating autoimmune and allergic diseases |
摘要 |
A process for obtaining human dendritic cells from monocytes (MODC) in the presence of interleukin-4 (IL-4), granulocyte macrophage colony stimulating factor (GM-CSF) and tumor necrosis factor alpha (TNF- alpha ) is new. A process for obtaining human dendritic cells from monocytes (MODC) in the presence of interleukin-4 (IL-4), granulocyte macrophage colony stimulating factor (GM-CSF) and tumor necrosis factor alpha (TNF- alpha ) is new and comprises several crucial technical parameters by cultivating the monocytes comprising: (a) the addition of 1-3 mg/ml bicarbonate to the culture medium; (b) maintaining the pH at 7.2-7.4; (c) culturing in Teflon pots; and (d) using 1-25% autologous/homologous human serum.
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申请公布号 |
FR2777906(A1) |
申请公布日期 |
1999.10.29 |
申请号 |
FR19980005584 |
申请日期 |
1998.04.27 |
申请人 |
ETABLISSEMENT DE TRANSFUSION SANGUINE DE LYON |
发明人 |
RIGAL DOMINIQUE;ELJAAFARI CORBIN ASSIA |
分类号 |
C12N5/0784;(IPC1-7):C12N5/08 |
主分类号 |
C12N5/0784 |
代理机构 |
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代理人 |
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地址 |
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