| 摘要 |
The invention concerns a cell line of human keratinocytes immortalised by at least a functional tumorigenic gene derived from a retrovirus characterised in that it is (1) non-tumorigenic, (2) preserves aptitude for differentiating and expressing proteins and enzymes which are expressed by normal differentiated keratinocytes even after a high rate of passages in tissue culture, and (3) forms a stratified and polarised epithelium comprising an ortho-keratotic stratum corneum, when cultivated in organotypical culture in a medium without serum and without feeder cell layer. The invention also concerns an improved method for immortalising human skin cells to obtain immortalised keratinocytes, characterised in that it comprises a step of infecting the keratinocytes using functional tumorigenic genes of at least two different retoviruses. The invention further concerns the use of said keratinocytes for immunologic, pharmacological, photo-toxicological and chemiotoxicological skin reactions for expressing heterologous genes and for constructing an artificial skin. |
