| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new protein comprising a protein Trad (salt) having a specific amino acid sequence, localized in skeleton skin and useful for prevention, treatment and diagnosis of diseases in skeletal muscle part such as muscular dystrophy and screening, etc., of the protein controller, etc. SOLUTION: This new protein comprises a new protein Trad (salt) containing substantially the same amino acid sequences as an amino acid sequence represented by the formula and has Db1 homologous (DH) region, a pleckstrin- like region and a phosphorylation enzyme region in single strand of polypeptide chain and is useful as a preventing agent and/or therapeutic agent for diseases in skeletal muscle part such as muscular dystrophy and/or useful for screening of the protein controller and diagnosis, etc., of diseases characteristics to skeletal muscle parts. The protein is obtained by searching EST database by using a base sequence of a human DAP kinase and carrying out PCR of human placenta cDNA library by using a synthetic oligonucleotide prepared from the resultant EST fragment as a primer, integrating the resultant Trad gene into a vector and expressing the gene. |
