HYBRIDOMA PRODUCING MONOCLONAL ANTIBODY OF ANTIGINSENOSIDE RB1

摘要

PROBLEM TO BE SOLVED: To provide the subject new cells that produces a monoclonal antibody to ginsenoside Rb1 with a specific molecular weight in a specific class and can simply and conveniently determine the ginsenoside Rb1 in various kinds of ginsengs and can closely examine many kinds of plants in a shortened time. SOLUTION: This is a monoclonal antibody to ginsenoside Rb1 with a molecular weight of 148,000, an IgG in the Ig G2b class and is a new hydridoma producing an antibody bearing theκlight chain. This is used in the usual ELISA to simply determine the content of ginsenoside Rb in variety of ginsengs, thus many kinds of plants can be closely examined. This hybridoma is obtained by immunizing an animal as a mouse with a ginsenoside Rg1-bovine serum albumen clathrate, then collecting the spleen cells and fusing them with myeloma cells and cloning then after cultivation in the HAT selection culture medium.