发明名称 |
Electrochemical sensor using intercalative, redox-active moieties |
摘要 |
Compositions and methods for electrochemical detection and localization of genetic point mutations and other base-stacking perturbations within oligonucleotide duplexes adsorbed onto electrodes and their use in biosensing technologies are described. An intercalative, redox-active moiety (such as an intercalator or nucleic acid-binding protein) is adhered and/or crosslinked to immobilized DNA duplexes at different separations from an electrode and probed electrochemically in the presence or absence of a non-intercalative, redox-active moiety. Interruptions in DNA-mediated electron-transfer caused by base-stacking perturbations, such as mutations or binding of a protein to its recognition site are reflected in a difference in electrical current, charge and/or potential. |
申请公布号 |
AU3550699(A) |
申请公布日期 |
1999.10.25 |
申请号 |
AU19990035506 |
申请日期 |
1999.04.07 |
申请人 |
CALIFORNIA INSTITUTE OF TECHNOLOGY |
发明人 |
JACQUELINE BARTON;MICHAEL HILL;SHANA KELLEY |
分类号 |
G01N33/50;C12Q1/68;G01N13/16;G01N27/416;G01N33/53;G01N37/00 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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