The invention relates to a method of nuclear transferring an isolated nucleus into an enucleated cell. In particular the donor nucleus is isolated by rupturing the cell membrane and aspiration using a tube having an internal diameter less than that of the donor cell and approximately the same or greater than that of the nucleus. The isolated nucleus is then transferred into an enucleated recipient oocyte by means of microinjection. The reconstituted cell thus obtained is transplanted as an embryo to produce an animal.
