CREATION OF THREE-DIMENSIONAL TISSUES

摘要

A method of providing a vascularized, three-dimensional tissue in a living subject is disclosed. The method includes the steps of (a) creating, from a biocompatible material capable of supporting cell adhesion, growth, and migration, a porous construct containing cells to be transplanted, and (b) delivering the construct into an area of interest in the living subject to form a vascularized three-dimensional tissue. The preferred construct has a dimension in which it is about 50 .mu.m to about 500 .mu.m from the outermost surface to the center of the construct. The preferred construct also has an interconnected porous structure having a pore size of from about 10 .mu.m to no greater than 300 .mu.m. The cells within the preferred construct are no greater than 250 .mu.m from an outer surface of the construct.