| 摘要 |
<p>A noncloning method for expressing a gene of interest in a mammalian host cell is disclosed. The invention utilizes three basic individual elements: 1) a promoter element; 2) at least one gene of interest; and 3) a selectable marker cassette which includes in 5' to 3' order, an internal ribosome entry site ('IRES'), at least one gene coding for a selectable marker, and a transcription termination sequence. The three individual elements are cotransfected into a mammalian host cell where they become operably linked such that expression of the selectable marker gene(s) necessarily requires coexpression of the gene of interest.</p> |
