| 摘要 |
Disclosed are methods for identifying genetic determinants that modulate the activity of test compounds which affect detectable processes in eukaryotic cells. The process comprises exposing a first cell, whether prokaryotic or eukaryotic, to a test compound to determine if the test compound modulates the detectable process in the cell. If so, the compound is then exposed to a second pool of cells (preferably of the same type as the first pool of cells) which overexpress one or more heterologous polypeptides under conditions which, in the absence of the compound, allow the detectable process to occur. If overexpression of the heterologous polypeptide alters modulation of the detectable process, such modulation allows the detectable process to be correlated with the test compound or with a particular compound and the overexpressed heterologous polypeptide, and the gene encoding the same. Also described are methods for correlating a gene with a detectable cellular process, wherein a first pool of cells, whether prokaryotic or eukaryotic, is exposed to a test compound, determining if the test compound modulates a detectable process in the cells, and if so, exposing the compound to a second pool of cells (preferably of the same type as the first pool of cells) which overexpress a heterologous polypeptide. If overexpression of the heterologous polypeptide alters modulation of the detectable process in the cells by the compound, the heterologous polypeptide responsible for such modulation, and preferably the nucleic acid molecule encoding the same, are characterized and correlated with the detectable process.
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