| 摘要 |
PCT No. PCT/JP96/01236 Sec. 371 Date Jan. 13, 1998 Sec. 102(e) Date Jan. 13, 1998 PCT Filed May 9, 1996 PCT Pub. No. WO96/35786 PCT Pub. Date Nov. 14, 1996A nacrein protein is expressed by separating a protein fraction having a molecular weight of sixty thousand, as determined by SDS-PAGE, from pearl proteins extracted from the nacreous layers of pearl oysters, synthesizing a probe coding for the N-terminal amino acid sequence of the protein in this fraction, cloning the cDNA derived from the mRNA of the mantle of the pearl oyster and hybridizable with the synthesized probe, and introducing the cloned cDNA into the host cells of Escherichia coli.
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