Method of plant tissue culture and regeneration

摘要

PCT No. PCT/GB94/02252 Sec. 371 Date Mar. 14, 1996 Sec. 102(e) Date Mar. 14, 1996 PCT Filed Oct. 14, 1994 PCT Pub. No. WO95/10178 PCT Pub. Date Apr. 20, 1995Plants may be regenerated from stomatal cells or protoplasts of such cells. Prior to regeneration the cells or protoplasts may be genetically transformed by the introduction of hereditary material most preferably by a DNA construct which is free of genes which specify resistance to antibiotics. The regeneration step may include callus formation on a hormone-free medium. The method is particularly suitable for sugar beet.