发明名称 |
Purified thermostable nucleic acid polymerase enzyme from thermosipho africanus |
摘要 |
PCT No. PCT/US91/07076 Sec. 371 Date Feb. 9, 1993 Sec. 102(e) Date Feb. 9, 1993 PCT Filed Sep. 26, 1991 PCT Pub. No. WO92/06202 PCT Pub. Date Apr. 16, 1992A purified thermostable enzyme is derived from the eubacterium Thermosipho africanus. The enzyme has DNA polymerase, activity reverse transcriptase activity, and optionally 5'->3' and/or 3'->5' exonuclease activity. The enzyme can be native or recombinant, and may be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
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申请公布号 |
US5968799(A) |
申请公布日期 |
1999.10.19 |
申请号 |
US19930977428 |
申请日期 |
1993.02.09 |
申请人 |
ROCHE MOLECULAR SYSTEMS, INC. |
发明人 |
GELFAND, DAVID H.;GREENFIELD, I. LAWRENCE;REICHERT, FRED L. |
分类号 |
C12N1/21;C12N9/12;C12N15/09;C12N15/54;C12R1/01;C12R1/19;(IPC1-7):C12N9/12 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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