| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new insersion sequence consisting of an insersion sequence having a specific nucleic acid sequence or a nucleic acid sequence complementary to the sequence, existing on a chromosome of E. coli and useful for e.g. rapid and easy classification of bacterial types, deletion of Vero toxin-producing capability of Vero toxin-producing E. coli. SOLUTION: This new insersion sequence is such one as to have a nucleic acid sequence expressed by the formula or a nucleic acid sequence complementary to the sequence and existing on a chromosome of E. coli. This new insersion sequence is useful for e.g. a method for amplifying one or more DNA fragments from DNA fragments in plural regions interposed in the insersion sequence, a rapid and easy method for classifying bacterial types through comparison of chain lengths of the amplified DNA fragments, a method for deleting Vero toxin-producing capability of Vero toxin-producing E. coli. This insersion sequence is obtained by amplifying a Vero toxin through PCR using an oligonucleotides having a specific base sequence as a primer so as to detect insersion of exogenous DNA fragments occurring in the Vero toxin gene, obtaining a DNA fragment having a large molecular weight and analyzing the base sequence of the resultant DNA fragment. |
