PIN METHOD FOR MEASURING BIOACTIVE SUBSTANCE

摘要

This present invention is for a method referred to as the pin method that greatly simplifies the detection and determination of the identity, amount and activity of molecules with biological activity. It is concerned with use of a device that will facilitate and simplify the measurement of the amount and activity of enzymes, enzyme inhibitors, lectins, receptors and other biologically active substances using a one-step technique for isolating a reactant from a product after completion of the reaction. The method involves the insertion of a pin-like device whose surface is coated with a reactant into a reaction vessel containing the appropriate compounds, allowing the reaction to proceed for a given period of time and then withdrawing the pin-like device from the reaction vessel. The amount of labeled products or reactants remaining in the reaction vessel can be directly measured without additional steps.