HUMANIZED ANTIBODY AGAINST HUMAN TISSUE FACTOR (TF) AND PROCESS FOR CONSTRUCTING HUMANIZED ANTIBODY

摘要

A humanized antibody against tissue factor (TF) which comprises: A. a humanized H chain containing (1) an H chain V region containing the H chain CDR of a mouse monoclonal antibody against TF and the H chain FR of a human antibody, and (2) the H chain C region of a human antibody; and B. a humanized L chain containing (1) an L chain V region containing the L chain CDR of a mouse monoclonal antibody against TF and the L chain FR of a human antibody, and (2) the L chain C region of a human antibody. The mouse monoclonal antibody CDR is grafted into the human antibody to construct the humanized V region. Next, the FR thereof is replaced by the corresponding FR of another human antibody with a high homology, thus detecting a highly active humanized antibody.