发明名称 |
Process for the in vivo recombination of DNA sequences having mismatched bases |
摘要 |
The present invention relates to a process of recombination in vivo of partially homologous DNA sequences having up to 30% of base mismatches. According to its essential characteristic, said sequences are placed together in cells or an organism of which the enzymatic mismatch repair system is defective or has been transitorily inactivated by saturation for the time to obtain recombination between said DNA sequences or in using mutants which increase the intergeneric recombination.
|
申请公布号 |
US5965415(A) |
申请公布日期 |
1999.10.12 |
申请号 |
US19950477473 |
申请日期 |
1995.06.07 |
申请人 |
MIXIS FRANCE, S.A. |
发明人 |
RADMAN, MIROSLAV;RAYSSIGUIER, CHRISTIANE |
分类号 |
C12N15/09;C12N1/21;C12N15/10;C12P21/00;C12R1/19;C12R1/42;C12R1/91;(IPC1-7):C12N15/64 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|