The three-dimensional structure of crystalline pertussis holotoxin (PT) has been determined by X-ray crystallography. Crystal structures have also been determined for complexes of pertussis toxin with molecules relevant to the biological activity of PT. These three-dimensional structures were analyzed to identify functional amino acids appropriate for modification to alter the biological properties of PT. Similar procedures may be used to predict amino acids which contribute to the toxicity of the holotoxin, to produce immunoprotective, genetically-detoxified analogs of pertussis toxin.
申请公布号
US5965385(A)
申请公布日期
1999.10.12
申请号
US19950467974
申请日期
1995.06.06
申请人
CONNAUGHT LABORATORIES LIMITED;UNIVERSITY OF ALBERTA
发明人
READ, RANDY J.;STEIN, PENELOPE E.;COCKLE, STEPHEN A.;OOMEN, RAYMOND P.;LOOSMORE, SHEENA;KLEIN, MICHEL H.;ARMSTRONG, GLEN D.;HAZES, BART