| 摘要 |
The present invention provides a method of creating expression libraries by marking desired clones with a unique tag. An expression library including individual clones marked with a unique tag sequence is also provided. Also provided is a method of isolating genetic elements associated with complete or partial cell growth suppression or cell death by isolating clones from an expression library which are lost in the cell population. A method of selection subtraction is provided by tagging a clone in an expression library, cloning the tagged clone into a vector, delivering the tagged clone to a target cell, and comparing tags before and after selection whereby toxic genes and the attached tags disappear. |
