发明名称 |
Expansion of bone marrow stromal cells |
摘要 |
The invention features a method for the selection and expansion of bone marrow stromal cells. The method includes the steps of obtaining bone marrow stromal cells; introducing the stromal cells into a vessel pre-coated on an inner surface with a gelatin, and containing a culture medium including an acidic fibroblast growth factor ("aFGF") polypeptide; and expanding the stromal cells in the culture medium under conditions and for a time sufficient to obtain an increased number of bone marrow stromal cells. The culture medium additionally can include heparin, and the vessel additionally can be precoated with fetal bovine serum.
|
申请公布号 |
US5962323(A) |
申请公布日期 |
1999.10.05 |
申请号 |
US19980094918 |
申请日期 |
1998.06.15 |
申请人 |
ALG COMPANY |
发明人 |
GREENBERGER, JOEL S.;HURWITZ, DAVID R. |
分类号 |
C12N5/02;C12N5/077;(IPC1-7):C12N5/06;C12N5/08;C07K15/50;C08B37/10 |
主分类号 |
C12N5/02 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|