发明名称 |
Functional characterization of genes |
摘要 |
Insertions into a gene of known sequence can be generated by crossing two parent plants, one of which contains a transposable element, to produce F1 progeny plants in which the insertion is detected by means of a PCR. F1 progeny plants containing such an insertion are self-fertilized to produce F2 progeny which are homozygous for the insertion. The function of a gene disabled by the insertion can be ascertained from a comparison of the phenotype of the F2 progeny with a parental phenotype. Large numbers of F1 progeny can be tested simultaneously for the presence of insertions. A collection of F2 seed can be stored and used for phenotype comparison when an insertion is detected.
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申请公布号 |
US5962764(A) |
申请公布日期 |
1999.10.05 |
申请号 |
US19970835638 |
申请日期 |
1997.04.10 |
申请人 |
PIONEER HI-BRED INTERNATIONAL, INC. |
发明人 |
BRIGGS, STEVEN P.;MEELEY, ROBERT B. |
分类号 |
A01H1/04;C12N15/82;C12Q1/68;(IPC1-7):A01H4/00;C12N15/09 |
主分类号 |
A01H1/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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