| 摘要 |
Secondary (hetero)arylmethylamine derivatives are used as chromogenic reagents in peroxidase/hydrogen peroxide assay systems. Assay for hydrogen peroxide, hydrogen peroxide-producing systems, peroxidases, oxidases or substances with peroxidase activity comprises using a chromogenic reagent of formula (I) and quantifying the resulting color change or colored precipitate: Z = carbon-bearing substituent with n C-N bonds, preferably an optionally substituted mono-, di- or trivalent aromatic or heteroaromatic group; n = 1 or more; a = 0 or 1; R4, R5 = H; R2, R3 = phenyl with 0-5 substituents; heteroaryl, e.g. pyridyl with 0-4 substituents or pyrrolyl, furyl or thienyl with 0-3 substituents; or metallocenyl, e.g. ferrocenyl with 0-9 substituents; where the substituents are selected from OH, alkoxy, aryloxy, acyloxy, alkyl, aryl, heteroaryl, halogen, NO2, COOH, alkoxycarbonyl, aryloxycarbonyl, NH2, aminoalkyl and aminoaryl; A = an anion. R1 = not defined.
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