发明名称 |
Non-toxic mutants of pathogenic gram-negative bacteria |
摘要 |
<p>A method is provided for identifying, isolating, and producing htrB mutants of gram-negative bacterial pathogens. The method comprises mutating the htrB gene of a gram-negative bacterial pathogen so that there is a lack of a functional htrB protein, resulting in a mutant that lacks one or more secondary acyl chains contained in the wild type gram-negative bacterial pathogen, and displays substantially reduced toxicity as compared to the wild type strain. Also, the present invention provides methods for using a vaccine formulation containing the htrB mutant, the endotoxin isolated therefrom, or the endotoxin isolated therefrom which is then conjugated to a carrier protein, to immunize an individual against infections caused by gram-negative bacterial pathogens by administering a prophylactically effective amount of the vaccine formulation.</p> |
申请公布号 |
AU710933(B2) |
申请公布日期 |
1999.09.30 |
申请号 |
AU19970011246 |
申请日期 |
1996.11.27 |
申请人 |
UNIVERSITY OF IOWA RESEARCH FOUNDATION;THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;AMERICAN CYANAMID COMPANY |
发明人 |
MICHAEL A. APICELLA;MELVIN G. SUNSHINE;LEE, NA-GYONG;RASAPPA ARUMUGHAM;BRADFORD W GIBSON |
分类号 |
C12N15/09;A61K39/00;A61K39/02;A61K39/09;A61K39/095;A61K39/102;A61K39/104;A61K39/106;A61K39/112;A61K39/116;A61P31/04;C12N1/21;C12N9/10;C12N15/01;C12R1/01;(IPC1-7):A61K35/66;C07K14/195 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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