发明名称 |
Methods for detecting differentially expressed genes |
摘要 |
<p>Disclosed is a method for detecting differentially expressed genes, which comprises the steps of: (a) providing a first sample of nucleic acids representing a first population of RNA transcripts and a second sample of nucleic acids representing a second population of RNA transcripts; (b) labeling the nucleic acids in the first sample with a first labeling element, and labeling the nucleic acids in the second sample with a second labeling element; (c) hybridizing the labeled nucleic acids in each sample to an excess of copies of a gene-specific sequence from a DNA library; (d) subjecting the hybridized nucleic acids in the first sample to a first enzymatic reaction and subjecting the hybridized nucleic acids in the second sample to a second enzymatic reaction, thereby generating a first chromogen from the first sample and generating a second chromogen from the second sample; and (e) determining the amounts of the first chromogen and the second chromogen relative to each other; wherein a difference in the amounts of the first chromogen and the second chromogen indicates that the gene-specific sequence is differentially expressed in the first population of RNA transcripts and the second population of RNA transcripts.</p> |
申请公布号 |
EP0945519(A2) |
申请公布日期 |
1999.09.29 |
申请号 |
EP19990104051 |
申请日期 |
1999.03.17 |
申请人 |
ACADEMIA SINICA |
发明人 |
PECK, KONAN;CHEN, JEREMY J. W.;YANG, PAN-CHYR;WU, REEN;CHANG, FU;CHU, YI-WEN;WU, CHENG-WEN |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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