METHOD OF PURIFYING CHROMOSOMAL DNA REPLICATION INITIATING PROTEIN DNAA OF PATHOGENIC BACTERIUM USING AFFINITY OF DNAA PROTEIN FOR ATP, METHOD OF EVALUATING ANTIBACTERIAL ACTIVITY AND METHOD OF SCREENING COMPOUND HAVING ANTIBACTERIAL ACTIVITY USING AFFINITY OF DNAA PROTEIN FOR ATP

摘要

DnaA protein of Staphylococcus aureus can be purified to a purity of 99% or higher from a crude extraction fraction by using ATP-binding activity as an indication. The thus purified DnaA protein shows a high affinity for ATP. Using the affinity of the purified DnaA protein for ATP, antibacterial activity can be evaluated and a compound having an antibacterial activity can be efficiently screened.