发明名称 |
Capillary electrophoresis enzyme immunoassay |
摘要 |
Disclosed are methods and apparatus for performing highly sensitive enzyme-amplified assays using channel electrophoresis of an enzyme having a biorecognition moiety. Combining a binding reaction of an analyte and an enzyme having a biorecognition moiety, and an electrophoretic separation which uses enzyme-amplified detection methodology, assay methods and apparatus are provided that offer simplicity, quantitative sensitivity and rapid analysis. Also disclosed are highly sensitive catalytically-amplified assays using a capillary electrophoresis format in which the binding reaction prior to electrophoresis is heterogeneous or homogeneous, and direct or competitive. The methods and apparatus for conducting sensitive automated catalytically-amplified assays using electrophoresis may be conducted in a microscale format.
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申请公布号 |
US5958202(A) |
申请公布日期 |
1999.09.28 |
申请号 |
US19970792933 |
申请日期 |
1997.01.22 |
申请人 |
PERSEPTIVE BIOSYSTEMS, INC. |
发明人 |
REGNIER, FRED E.;YAO, XIAN-WEI;TAYLOR, TODD A.;FUCHS, MARTIN;SCHMALZING, DIETER;KOUTNY, LANCE;NASHABEH, WASSIM |
分类号 |
G01N33/561;G01N27/447;G01N33/537;G01N33/543;G01N33/78;(IPC1-7):G01N27/26;G01N33/53;C12Q1/68 |
主分类号 |
G01N33/561 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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