| 摘要 |
A purified preparation of type II porcine circovirus (PCV). Independent claims are also included for the following: (a) preparation of PCV (i) isolated from a physiological or tissue sample, particularly from a lesion, from a pig with symptoms of PMWS (porcine multisystemic wasting syndrome); or (ii) produced by, and isolated from, in vitro cell cultures infected with the virus of (i); (b) extract or culture supernatant, or antigen preparation, optionally purified, collected from in vitro cultures of cells infected with PCV; (c) vaccine containing the products of (b); (d) DNA fragments (A) of 1767 bp (1), 1767 bp (2), 1767 bp (3), 1768 bp (4) or 1768 bp (6), or containing an open reading frame (ORF) of PCV; (e) polypeptides (I) encoded by (A) or these ORF; in vitro expression vector containing (A), or these ORFs; (f) polypeptides (Ia), optionally purified, expressed from the vector of (f); (g) subunit vaccine containing at least one (I) or (Ia), diluent or vehicle and optionally an adjuvant; in vivo expression vector, integrated into a genome, containing (A) or the ORFs; (h) live or plasmid vaccine containing the vector of (j), and a diluent or vehicle; (i) probe or primer containing all or part of (A) or the ORFs; (j) mono- or poly-clonal antibodies raised against PCV, (I), (Ia) or their fragments; and (k) detection of PCV by identifying in a body fluid or tissue sample an antigen or antibody specific for the antigen.
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