发明名称 |
Non-infectious, replication-defective, self-assembling HIV-1 viral particles containing antigenic markers in the gag coding region |
摘要 |
The present invention is concerned with the ability to differentiate between infection by HIV and immunization with an immunogenic preparation. Accordingly, the present invention provides a non-infectious, non-replicating, HIV retrovirus-like particle containing a heterologous antigenic marker, comprising an assembly of (a) an env gene product; (b) a pol gene product; (c) a gag gene product; and, (d) at least one non-retroviral, non-mammalian antigenic marker. The antigenic marker may have between 5 and 100 amino acid residues. In one embodiment, the amino acid sequence contains a tobacco mosaic virus (TMV) epitope obtained from the coat protein. In another embodiment, the marker is inserted into the gag gene product at an antigenically-active insertion site. The presence of the antigenic marker enables recognition that antiserum containing anti-HIV antibodies has been generated by exposure to the non-infectious retrovirus-like particles, as opposed to wild-type HIV infection, by testing for antibodies specific to the antigenic marker.
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申请公布号 |
US5955342(A) |
申请公布日期 |
1999.09.21 |
申请号 |
US19940290105 |
申请日期 |
1994.08.15 |
申请人 |
CONNAUGHT LABORATORIES LIMITED |
发明人 |
ROVINSKI, BENJAMIN;CAO, SHI-XIAN;YAO, FEI-LONG;PERSSON, ROY;KLEIN, MICHEL H. |
分类号 |
A61K39/00;A61K39/21;A61K39/39;C07K14/08;C07K14/11;C07K14/16;C12N7/00;C12N7/04;C12N15/09;C12P21/08;C12Q1/68;G01N33/569;(IPC1-7):C12N7/04;A61K39/12 |
主分类号 |
A61K39/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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