发明名称 |
IMPROVED METHODS FOR PRODUCING FACTOR VIII PROTEINS |
摘要 |
Methods are provided for purification of Factor VIII polypeptides by immunoaffinity chromatography and ion exchange chromatography, in which the eluate from the immunoaffinity column is diluted with a solution comprising higher salt concentration, or lower non-polar agent concentration than that of the elution solution, prior to passing the diluted solution through the ion exchange column. The methods result in improved purification without significant yield loss.
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申请公布号 |
WO9946299(A1) |
申请公布日期 |
1999.09.16 |
申请号 |
WO1999US05329 |
申请日期 |
1999.03.11 |
申请人 |
GENETICS INSTITUTE, INC. |
发明人 |
KELLEY, BRIAN, D.;DALBORD, JOHANNA;PETERSSON, ANNA |
分类号 |
C12N15/09;C07K14/755;(IPC1-7):C07K14/755 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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