发明名称 |
ENZYMATIC PREPARATION PROCESS OF 7{BETA}(4-CARBOXYBUTANAMIDO)CEPHALOSPORANIC ACID BY MAKING USE OF MODIFIED OXIDASE OF D-AMINO ACIDS FROM TRIGONOPSIS VARIABILIS PRODUCED IN ESCHERICHIA COLI |
摘要 |
Enzymatic process for the preparation of cephalosporanic 7 beta -(4-carboxybutanamide) acid by using the modified enzyme D-aminoacid oxidase of Trigonopsis variabilis produced in Escherichia coli. The process for the expression of the enzyme comprises: (I) isolating the DNA corresponding to the gene which codes for the enzyme D-aminoacide oxidase; (II) removing the intron which is contained in said gene; (III) inserting the DNA fragment obtained into the plasmide which is capable of replication in Escherichia coli; (IV) fusing at the extremity 5' of the structural region of the gene a synthetic assembler which contains a nucleotide sequence which codes for six histidines; (V) transforming a strain of Escherichia coli with the resulting recombinant plasmide; (VI) cultivating the transformed cells of Escherichia coli; and (VII) recovering the enzyme D-aminoacid oxidase of the former cultivation operation through affinity chromatography. <IMAGE> |
申请公布号 |
CZ9901823(A3) |
申请公布日期 |
1999.09.15 |
申请号 |
CZ19990001823 |
申请日期 |
1998.09.24 |
申请人 |
ANTIBIOTICOS |
发明人 |
GARCIA LOPEZ JOSE LUIS;CORTES RUBIO ESTRELLA;ALONSO PALACIOS JORGE;MELLADO DURAN ENCARNACION;DIEZ GARCIA BRUNO;GUISAN SEIJAS JOSE MANUEL;SALTO MALDONADO FRANCISCO;BARREDO FUENTE JOSE LUIS |
分类号 |
C12N15/09;C12N1/21;C12N9/06;C12P17/18;C12P35/06;C12R1/01 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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